Phosphorylation is an important biochemical process that underpins almost all cellular behaviour. This makes it an incredible tool in understanding how cells work and respond to drugs. This research aims to leverage phosphoproteomics to understand how a library of 30,000 small drug-like compounds effect stem cells. Characterising a library this big is unprecedented using phosphoproteomics, thus our lab has turned to using robotics to automate our sample preparation.
Impact
Once completed, this research will provide an invaluable resource for scientists. reNEW researchers will be able to access the database of characterised compounds to screen thousands of drugs and focus on the most promising drugs in their stem cell models. This approach will accelerate the development of more effective and targeted treatments for multiple diseases.
reNEW research
Dr Sean Humphrey and his team have developed automated robotic phosphoproteomic sample preparation pipelines, integrating them seamlessly with cutting-edge mass spectrometry technologies and advanced machine learning techniques to identify potentially therapeutic compounds within the reNEW library.
Image description
Our robotic arm highlighted with an LED strip, as it moves though its sample preparation steps, assisting in the drugging of induced pluripotent stem cells.
Credits
Jack Leerson, Research Assistant, Functional Phosphoproteomics lab members, Functional Phosphoproteomics Lab, reNEW Melbourne.
